Trincomalee Campus

RESEARCH LABORATORY

AUTO CLAVE MACHINE

This machine is available in the microbiology laboratory. Autoclaves use pressurized steam as their sterilization agent. Mostly it is used to sterilize glassware, culture media Liquid Media, Non-flammable liquids, Aqueous solutions and Liquid biological waste. The amount of time and temperature required for sterilization depends on the type of material being autoclaved. Using higher temperatures for sterilization requires shorter times. The most common temperatures used are 1210 C and 1320 C. In order for steam to reach these high temperatures, steam has to be pumped into the chamber at a pressure higher than normal atmospheric pressure.

INCUBATOR

It is available in the Microbiology laboratory. Incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the CO (CO2) and oxygen content of the atmosphere inside. Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eukaryotic cells.

ORBIT SHAKER

An orbital shaker is available in the Microbiology laboratory has a circular shaking motion with a slow speed (25-500 rpm). It is suitable for culturing microbes, washing blots, and general mixing. Some of its characteristics are that it does not create vibrations, and it produces low heat compared to other kinds of shakers, which makes it ideal for culturing microbes. Moreover, it can be modified by placing it in an incubator to create an incubator shaker due to its low temperature and vibrations.

HOT AIR OVEN STERILIZER

Hot air ovens are electrical devices available at the Microbiology laboratory which use dry heat to sterilize. They were originally developed by Pasteur. Generally, they can be operated from 50 to 300 °C, using a thermostat to control the temperature. These are widely used to sterilize articles that can withstand high temperatures and not get burnt, like glassware and powders. Linen gets burnt and surgical sharps lose

BAG MIXER

Avoiding all risks of cross-contamination, Bag Mixer is available at the Microbiology laboratory. It is easy to use and powerful lab blender. Adapted to all kinds of applications and with a guarantee of optimal bacterial extraction, it is a great tool for lab analyses.

MUFFLE FURNACE

Muffle furnace is a closed chamber laboratory instrument used for heating at high temperature ranges from 50°C to 1800°C.  Zirconia Fiber Board/Alumina Bricks gives an advantage to the high temperature furnace chamber. It is called as a box furnace because of its design and shape & Muffle furnace because of its working without noise.

HOT AIR OVEN

A hot air oven is available in the research laboratory. It is a type of dry heat sterilization. Dry heat sterilization is used on equipment that cannot be wet and on material that will not melt, catch fire, or change form when exposed to high temperatures. Moist heat sterilization uses water to boil items or steam them to sterilize and doesn’t take as long as dry heat sterilization. Examples of items that aren’t sterilized in a hot air oven are surgical dressings, rubber items, or plastic material.
Items that are sterilized in a hot air oven include:
• Glassware (like petri dishes, flasks, pipettes, and test tubes)
• Powders (like starch, zinc oxide, and sulfadiazine)
• Materials that contain oils
• Metal equipment (like scalpels, scissors, and blades)
Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. The ovens use conduction to sterilize items by heating the outside surfaces of the item, which then absorbs the heat and moves it towards the center of the item. The commonly-used temperatures and time that hot air ovens need to sterilize materials is 170 degrees Celsius for 30 minutes, 160 degrees Celsius for 60 minutes, and 150 degrees Celsius for 150 minutes.

BIO-SAFETY CABINET

Bio safety cabinet is available at the Microbiology Laboratory. These are among the most effective primary containment devices used in laboratories working with infectious agents. They act as primary barriers to prevent the escape of biological aerosols into the laboratory environment. This is important because most laboratory techniques (e.g.; centrifuging, pipetting, vortexing, sonicating) produce inadvertent aerosols that can be readily inhaled by the researcher.