Trincomalee Campus

RESEARCH LABORATORY

BIO-SAFETY CABINET

Bio safety cabinet is available at the Microbiology Laboratory. These are among the most effective primary containment devices used in laboratories working with infectious agents. They act as primary barriers to prevent the escape of biological aerosols into the laboratory environment. This is important because most laboratory techniques (e.g.; centrifuging, pipetting, vortexing, sonicating) produce inadvertent aerosols that can be readily inhaled by the researcher.

FREEZE DRYER

Freeze dryer is available at the Biochemistry Laboratory. Freeze dryer, designed for routine applications. It has a maximum ice capacity of 4 kg, with ice condenser temperature of -85 °C that permits drying of samples with organic solvents such as alcohols. It is used to increase the shelf life of the products, such as live virus vaccines, biologics and other injectable. By removing the water from the material and sealing the material in a glass vial, the material can be easily stored, shipped, and later reconstituted to its original form for injection.



GAS- CHROMATOGRAPHY-MASS SPECTRUM

This instrument is available at our Biochemistry laboratory. Gas chromatography–mass spectrometry (GC-MS) is an analytical machine that composed of two major building blocks: the gas chromatograph and the mass spectrometer. It used to identify different substances within a test sample. Our research students used to investigate GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, and identification of unknown samples.




ORBIT SHAKER

An orbital shaker is available in the Microbiology laboratory has a circular shaking motion with a slow speed (25-500 rpm). It is suitable for culturing microbes, washing blots, and general mixing. Some of its characteristics are that it does not create vibrations, and it produces low heat compared to other kinds of shakers, which makes it ideal for culturing microbes. Moreover, it can be modified by placing it in an incubator to create an incubator shaker due to its low temperature and vibrations.

INCUBATOR

It is available in the Microbiology laboratory. Incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the CO (CO2) and oxygen content of the atmosphere inside. Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eukaryotic cells.

MUFFLE FURNACE

Muffle furnace is a closed chamber laboratory instrument used for heating at high temperature ranges from 50°C to 1800°C. Zirconia Fiber Board/Alumina Bricks gives an advantage to the high temperature furnace chamber. It is called as a box furnace because of its design and shape & Muffle furnace because of its working without noise.

DRYING CABINET

Drying cabinet is available in our Physiology Laboratory. Originally designed for drying Pyrex laboratory glassware and stainless steel surgical instruments, LEEC drying cabinets can be used to dry pretty much anything that cannot be dried in a laboratory oven (lab ovens tend to dry at temperatures higher than +100°C). Drying cabinets can dry at up to a maximum of 50°C above room temperature.

HOT AIR OVEN STERILIZER

Hot air ovens are electrical devices available at the Microbiology laboratory which use dry heat to sterilize. They were originally developed by Pasteur. Generally, they can be operated from 50 to 300 °C, using a thermostat to control the temperature. These are widely used to sterilize articles that can withstand high temperatures and not get burnt, like glassware and powders. Linen gets burnt and surgical sharps lose

BAG MIXER

Avoiding all risks of cross-contamination, Bag Mixer is available at the Microbiology laboratory. It is easy to use and powerful lab blender. Adapted to all kinds of applications and with a guarantee of optimal bacterial extraction, it is a great tool for lab analyses.

DE-IONIZER

De-ionizer is available at our research laboratory. It is used to de-ionize the distil water collected.

DISTILL WATER PLANT

This machine is available at the physiology laboratory. It is used to purify the tap water out of contaminants to be used for the research works.

MICROWAVE DIGESTER

Microwave is a technique used to dissolve solid sample matrices into an aqueous liquid. This is achieved by placing a sample in a concentrated acid matrix in a closed vessel and exposing it to microwave irradiation. Both the speed of thermal decomposition of the sample and the solubility of metals are increased. Once these metals are in solution, they can be quantified through spectroscopic techniques. This is available in our Biochemistry Laboratory.

AUTO CLAVE MACHINE

This machine is available in the microbiology laboratory. Autoclaves use pressurized steam as their sterilization agent. Mostly it is used to sterilize glassware, culture media Liquid Media, Non-flammable liquids, Aqueous solutions and Liquid biological waste. The amount of time and temperature required for sterilization depends on the type of material being autoclaved. Using higher temperatures for sterilization requires shorter times. The most common temperatures used are 1210 C and 1320 C. In order for steam to reach these high temperatures, steam has to be pumped into the chamber at a pressure higher than normal atmospheric pressure.

CENTRIFUGATION MACHINE

Centrifuge is a laboratory device available at our Microbiology, Biochemistry and Physiology laboratories. It is used for the separation of fluids, gas or liquid, based on density. Separation is achieved by spinning a vessel containing material at high speed; the centrifugal force pushes heavier materials to the outside of the vessel. This apparatus is found in most laboratories from academic to clinical research and used to purify cells, subcellular organelles, viruses, proteins, and nucleic acids. There are multiple types of centrifuge, which can be classified by intended use or by rotor design. From the large floor variety to the micro-centrifuge, there are many varieties available for the researcher.

HEMAOCYTTOMETER

Hemocytometer is available at the Physiology laboratory and consists of a thick glass microscope slide with a rectangular indentation that creates a chamber. This chamber is engraved with a laser-etched grid of perpendicular lines. The device is carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. By observing a defined area of the grid, it is therefore possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall.

WATER BATH

Water bath is available at our Biochemistry, Microbiology and Physiology laboratories. It is an equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time. All water baths have a digital or an analogue interface to allow users to set a desired temperature. Utilizations include warming of reagents, melting of substrates or incubation of cell cultures. It is also used to enable certain chemical reactions to occur at high temperature. Water bath is a preferred heat source for heating flammable chemicals instead of an open flame to prevent ignition.

HOT AIR OVEN

A hot air oven is available in the research laboratory. It is a type of dry heat sterilization. Dry heat sterilization is used on equipment that cannot be wet and on material that will not melt, catch fire, or change form when exposed to high temperatures. Moist heat sterilization uses water to boil items or steam them to sterilize and doesn't take as long as dry heat sterilization. Examples of items that aren't sterilized in a hot air oven are surgical dressings, rubber items, or plastic material. Items that are sterilized in a hot air oven include:
• Glassware (like petri dishes, flasks, pipettes, and test tubes)
• Powders (like starch, zinc oxide, and sulfadiazine)
• Materials that contain oils
• Metal equipment (like scalpels, scissors, and blades)
Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. The ovens use conduction to sterilize items by heating the outside surfaces of the item, which then absorbs the heat and moves it towards the center of the item. The commonly-used temperatures and time that hot air ovens need to sterilize materials is 170 degrees Celsius for 30 minutes, 160 degrees Celsius for 60 minutes, and 150 degrees Celsius for 150 minutes.

VORTEX MACHINE

A vortex mixer, is a simple device available in Biochemistry, Microbiology and Physiology laboratories. It is used commonly in laboratories to mix small vials of liquid. It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center. As the motor runs the rubber piece oscillates rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the motion is transmitted to the liquid inside and a vortex is created. Most vortex mixers are designed with 2 or 4-plate formats, have variable speed settings ranging from 100 to 3,200 rpm, and can be set to run continuously, or to run only when downward pressure is applied to the rubber piece.

HEMATOLOGY ANALYZER

This machine is available at the Physiology laboratory. Hematology analyzers are used to run tests on blood samples. They are used in the medical field to do white blood cell counts, complete blood counts, reticulocyte analysis, and coagulation tests. There are hematology analyzers that are for human blood or animal blood, which is useful for veterinarians, zoos, and research labs. Features vary from one hematology analyzer to another, such as closed vial testing and open sampling testing. Certain hematology analyzers allow the user to choose the preferred testing type. Other features to consider in a hematology analyzer are the sample size required, type and quantity of testing modes, speed in which the results are available, automatic flagging of results that are out of normal range, and the capacity of test results it can store.

FREEZERS

Ultra-freezer is available at the research laboratory. Laboratory freezers provide indispensable storage space for everything from bulk reagents to delicate tissue samples. Lab freezers can be chest format or upright. They are typically equipped with adjustable shelves designed to accommodate multiple types of sample containers. Programmable thermostats and temperature monitor systems, as well as alarms that sound in cases of power failure are common features. Standard freezers maintain a temperature just below freezing. Ultra low freezers, capable of temperatures of -80 degrees Celsius or lower, are available and often used for high-value samples.